The paper describes the process to produce Chitin Nanofibril-Hyaluronan\nnanoparticles (CN-HA), showing their ability to easily load active ingredients, facilitate\npenetration through the skin layers, and increase their effectiveness and safety as an anti-aging agent. Size and characterization of CN-HA nanoparticles were determined by\nScanning Electron Microscopy (SEM) and Zetasizer, while encapsulation efficiency and\nloading capacity of the entrapped ingredients were controlled by chromatographic and\nspectrophotometric methods. Safeness was evidenced on fibroblasts and keratinocytes\nculture viability by the MTT (Methylthiazol) assay; anti-aging activity was evaluated in vitro\nmeasuring antioxidant capacity, anti-collagenase activity, and metalloproteinase and\npro-inflammatory release; efficacy was shown in vivo by a double-blind vehicle-controlled\nstudy for 60 days on 60 women affected by photo-aging. In addition, the CN-HA\nnanoparticles have shown interesting possibility to be used as active ingredients, for\ndesigning and making advanced medication by the electrospinning technology, as well as\nto produce transparent films for food packaging, by the casting method, and can be used\nalso in their dry form as tissues or films without adding preservatives. These unusual\nCN-HA nanoparticles obtained from the use of raw materials of waste origin may offer an\nunprecedented occasion for making innovative products, ameliorating the quality of life,\nreducing pollution and safeguarding the environment�s integrity.
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